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A new molecular beacon (MB) in which fluorescein (FAM) attached to its 3′ end acts as a fluorophore and a bridged thymidine–Hg–thymidine (T–Hg–T) complex acts as a quencher is designed. The fluorescence resonance energy transfer (FRET) between the fluorophore and the quencher results in annihilation of the FAM fluorescence. Experimental conditions that govern the fluorescence quenching, such as number...
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