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Background Rice endosperm is composed of aleurone cells in the outermost layers and starchy endosperm cells in the inner part. The aleurone layer accumulates lipids, whereas starchy endosperm mainly accumulates starch. During the ripening stage, the starch accumulation rate is known to be asynchronous, depending on the position of the starchy endosperm. Different physiological and molecular mechanisms...
Laser microdissection (LMD) is now becoming a powerful tool to elucidate the spatial profiling of gene expression. However, a remaining difficulty is obtaining high integrity RNA from fixed and embedded tissues in order to obtain accurate and reliable expression analyses. This study aimed to develop methods for the preparation of high integrity RNA from aleurone cells and starchy endosperm of developing...
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