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Objective: To determine the incidence of nondisjunction for chromosomes X, Y, and 18 using fluorescence in situ hybridization (FISH) on morphologically normal sperm from infertile men who are candidates for ICSI.Design: After standard hematoxylin staining, sperm with normal morphology were identified using Kruger's strict morphology criteria. The location of each normal-appearing sperm was recorded...
Objective: To design a system for teaching intracytoplasmic sperm injection (ICSI) and to provide a standardized method to assess technical competency.Setting: University andrology laboratory.Design: Prospective study of method for training ICSI and prediction of ICSI outcome.Patient(s): Male infertility candidates for ICSI and fertile donors.Intervention(s): Sperm from 14 fertile donors and 21 oligospermic...
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