Search results for: H. Rodriguez-Martinez

Items from 1 to 20 out of 106 results

article

Redox cycling induces spermptosis and necrosis in stallion spermatozoa while the hydroxyl radical (OH•) only induces spermptosis

P Martín Muñoz, L Anel‐López, JM Ortiz‐Rodríguez, M Álvarez, more

Reproduction in Domestic Animals > 53 > 1 > 54 - 67

Oxidative stress is a major factor explaining sperm dysfunction of spermatozoa surviving freezing and thawing and is also considered a major inducer of a special form of apoptosis, visible after thawing, in cryopreserved spermatozoa. To obtain further insights into the link between oxidative stress and the induction of apoptotic changes, stallion spermatozoa were induced to oxidative stress through...

article

Flow cytometry in Spermatology: A bright future ahead

C Ortega‐Ferrusola, MC Gil, H Rodríguez‐Martínez, L Anel, more

Reproduction in Domestic Animals > 52 > 6 > 921 - 931

Techniques such as mass spectrometry have led to unprecedented knowledge of the proteins that are present in the spermatozoa of humans and other mammals. However, in spite of their high‐throughput and fractioning techniques, most of the techniques in use only offer average values for the entire sperm population. Yet, ejaculate is very heterogeneous, and average values may mask relevant biological...

article

Stallion spermatozoa surviving freezing and thawing experience membrane depolarization and increased intracellular Na⁺

C. Ortega Ferrusola, L. Anel‐López, J. M. Ortiz‐Rodriguez, P. Martin Muñoz, more

Andrology > 5 > 6 > 1174 - 1182

In order to gain insight of the modifications that freezing and thawing cause to the surviving population of spermatozoa, changes in the potential of the plasma membrane (Em) and intracellular Na⁺ content of stallion spermatozoa were investigated using flow cytometry. Moreover, caspase 3 activity was also investigated and the functionality of the Na⁺‐K⁺ ATPase pump was investigated before and after...

article

Peroxidized mineral oil increases the oxidant status of culture media and inhibits in vitro porcine embryo development

C.A. Martinez, A. Nohalez, J.J. Ceron, C.P. Rubio, more

Theriogenology > 2017 > 103 > C > 17-23

The use of oils with undetected alterations is a long-recognized problem for in vitro embryo production systems. Since peroxides in oils have been associated with reduced embryo production outcomes, our goals were (1) to evaluate the effects of a batch of mineral oil (MO) that was suspected to be altered on the in vitro production of pig embryos and (2) to determine oil peroxide values throughout...

article

The CatSper channel modulates boar sperm motility during capacitation

A. Vicente-Carrillo, M. Alvarez-Rodriguez, H. Rodriguez-Martinez

Reproductive Biology > 2017 > 17 > 1

article

Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows

C.A. Martinez, A. Nohalez, I. Parrilla, J.L. Vazquez, more

Theriogenology > 2017 > 87 > C > 316-320

Recent advances in nonsurgical deep uterine (NsDU) embryo transfer (ET) technology allow the noninvasive transfer of porcine embryos into recipients, overcoming the most important impediment for commercial ET in this species. Although many factors in the porcine ET-field have been recently evaluated, many others remain to be explored. We investigated here the future reproductive performance of donors...

article

Membrane Stress During Thawing Elicits Redistribution of Aquaporin 7 But Not of Aquaporin 9 in Boar Spermatozoa

A Vicente‐Carrillo, H Ekwall, M Álvarez‐Rodríguez, H Rodríguez‐Martínez

Reproduction in Domestic Animals > 51 > 5 > 665 - 679

Freezing of boar spermatozoa includes the cryoprotectant glycerol, but renders low cryosurvival, owing to major changes in osmolarity during freezing/thawing. We hypothesize that aquaporins (AQPs) 7 and 9 adapt their membrane domain location to these osmotic challenges, thus maintaining sperm homeostasis. Western blotting (WB) and immunocytochemistry (ICC) at light and electron microscope levels with...

article

Immunolocalization of E-cadherin and β-catenin in the cyclic and early pregnant canine endometrium

R. Payan-Carreira, M.A. Pires, C. Santos, B. Ström Holst, more

Theriogenology > 2016 > 86 > 4 > 1092-1101

Putative changes in E-cadherin and β-catenin during implantation in dogs are of interest to study, as they are relevant proteins for epithelial integrity. E-cadherin and β-catenin were immunolocalized in the canine endometrium during the estrous cycle and early pregnancy, using monoclonal antibodies. Both proteins were detected in all types of endometrial epithelia (surface epithelium [SE], superficial...

article

Will AI in pigs become more efficient?

J. Roca, I. Parrilla, A. Bolarin, E.A. Martinez, more

Theriogenology > 2016 > 86 > 1 > 187-193

AI is commercially applied worldwide to breed pigs, yielding fertility outcomes similar to those of natural mating. However, it is not fully efficient, as only liquid-stored semen is used, with a single boar inseminating about 2000 sows yearly. The use of liquid semen, moreover, constrains international trade and slows genetic improvement. Research efforts, reviewed hereby, are underway to reverse...

article

The ubiquitous hyaluronan: Functionally implicated in the oviduct?

H. Rodriguez-Martinez, P. Tienthai, M. Atikuzzaman, A. Vicente-Carrillo, more

Theriogenology > 2016 > 86 > 1 > 182-186

Hyaluronan (hyaluronic acid) is a simple, nonantigenic, nonsulfated glycosaminoglycan present everywhere in the extracellular compartments of the body. Noteworthy, it is highly conserved phylogenetically, from sauropsida to mammals; and plays a plethora of roles from embryonic/fetal development to adult physiological and pathological events, including tumor development. In reproduction, hyaluronan...

article

Non-viable sperm in the ejaculate: Lethal escorts for contemporary viable sperm

J. Roca, I. Parrilla, M.A. Gil, C. Cuello, more

Animal Reproduction Science > 2016 > 169 > C > 24-31

Non-viable sperm (“dead sperm”) are present in variable numbers in mammalian ejaculates and their number increase substantially when semen is stored, particularly cryopreserved. This review comparatively highlights, with experimental data in porcine, the role-played by non-viable sperm in the outcome of semen used in assisted reproductive technologies. As well, the review discusses our current understanding...

article

The role of placental-decidual interactions in promoting fetal tolerance

J. Ernerudh, G. Berg, M.C. Jenmalm, G.E. Lash, more

Journal of Reproductive Immunology > 2016 > 115 > C > 42

article

Flow Cytometric Chromosomal Sex Sorting of Stallion Spermatozoa Induces Oxidative Stress on Mitochondria and Genomic DNA

CM Balao da Silva, C Ortega‐Ferrusola, JM Morrell, H Rodriguez Martínez, more

Reproduction in Domestic Animals > 51 > 1 > 18 - 25

To date, the only repeatable method to select spermatozoa for chromosomal sex is the Beltsville sorting technology using flow cytometry. Improvement of this technology in the equine species requires increasing awareness of the modifications that the sorting procedure induces on sperm intactness. Oxidative stress is regarded as the major damaging phenomenon, and increasing evidence regards handling...

article

Boar Differences In Artificial Insemination Outcomes: Can They Be Minimized?

J Roca, MLWJ Broekhuijse, I Parrilla, H Rodriguez‐Martinez, more

Reproduction in Domestic Animals > 50 > S2 > 48 - 55

In Western countries, where pig breeding and production are intensive, there is a documented variability in fertility between farms with boar‐related parameters only accounting to 6% of this total variation of in vivo fertility. Such low boar effect could be a result of the rigorous control of sires and ejaculates yielding AI‐doses exerted by the highly specialized AI‐centres that monopolize the market...

article

The activity of paraoxonase type 1 (PON‐1) in boar seminal plasma and its relationship with sperm quality, functionality, and in vivo fertility

I. Barranco, A. Tvarijonaviciute, C. Perez‐Patiño, D. V. Alkmin, more

Andrology > 3 > 2 > 315 - 320

Paraoxonase 1 (PON‐1) is a hydrolytic enzyme present in body fluids, capable of protecting cells against oxidative stress. The hypothesis was hereby to test that PON‐1, present in seminal plasma (SP), acts protecting boar spermatozoa when showing a reasonable high activity in the ejaculate. SP‐PON‐1 activity differed (p < 0.001) among boars (from 0.10 to 0.29 IU/mL). Intra‐boar variability was...

article

Boar spermatozoa successfully predict mitochondrial modes of toxicity: Implications for drug toxicity testing and the 3R principles

A. Vicente-Carrillo, I. Edebert, H. Garside, I. Cotgreave, more

Toxicology in Vitro > 2015 > 29 > 3 > 582-591

Replacement of animal testing by in vitro methods (3-R principles) requires validation of suitable cell models, preferably obtained non-invasively, defying traditional use of explants. Ejaculated spermatozoa are highly dependent on mitochondrial production and consumption of ATP for their metabolism, including motility display, thus becoming a suitable model for capturing multiple modes of action...

article

Effect of Overnight Staining on the Quality of Flow Cytometric Sorted Stallion Sperm: Comparison with Tradtitional Protocols

CM Balao da Silva, C Ortega Ferrusola, JM Gallardo Bolaños, M Plaza Dávila, more

Reproduction in Domestic Animals > 49 > 6 > 1021 - 1027

Flow cytometry is considered the only reliable method for the separation of X and Y chromosome bearing spermatozoa in equines. The MoFlo SX DP sorter is highly efficient, allowing the production of foals of the desired sex. However, to achieve acceptable pregnancy rates the currently used protocol requires working with fresh semen obtained close to, or at, the sorting facility. An alternative protocol...

article

Colloid Centrifugation Selects Normal Spermatozoa from Polymorphic Bull Ejaculates: A Case Study

JM Morrell, H Rodriguez‐Martinez, M Andersson

Reproduction in Domestic Animals > 49 > 2 > 281 - 284

Semen from a Western Finncattle bull exhibiting a highly polymorphic spermiogram was processed by colloid centrifugation using Androcoll‐B, a species‐specific silane‐coated silica colloid. In the first experiment, Single Layer Centrifugation (SLC) was used to identify which density colloids were needed to separate different cell populations. Colloids of the two chosen densities were then used in a...

article

Sperm chromatin structure and sperm morphology: Their association with fertility in AI-dairy Ayrshire sires

S. Nagy, A. Johannisson, T. Wahlsten, R. Ijäs, more

Theriogenology > 2013 > 79 > 8 > 1153-1161

Prognostic relations between sperm variables and sire fertility are yet elusive. A retrospective analysis of sperm morphology and chromatin stability (studied using sperm chromatin structure assay [SCSA]) and their relation to fertility after AI (as proportions of 60 days of nonreturn to estrous [NRR], corrected NRR, or calving rate) was studied with preselected frozen semen doses from a group (N...

article

Sex sorting increases the permeability of the membrane of stallion spermatozoa

C.M. Balao da Silva, C. Ortega Ferrusola, A. Morillo Rodriguez, J.M. Gallardo Bolaños, more

Animal Reproduction Science > 2013 > 138 > 3-4 > 241-251

At present, the only repeatable means of selecting the sex of offspring is the Beltsville semen sorting technology using flow cytometry (FC). This technology has reached commercial status in the bovine industry and substantial advances have occurred recently in swine and ovine species. In the equine species, however, the technology is not as well developed. To better understand the changes induced...

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Search results for: H. Rodriguez-Martinez

Redox cycling induces spermptosis and necrosis in stallion spermatozoa while the hydroxyl radical (OH•) only induces spermptosis

Flow cytometry in Spermatology: A bright future ahead

Stallion spermatozoa surviving freezing and thawing experience membrane depolarization and increased intracellular Na⁺

Peroxidized mineral oil increases the oxidant status of culture media and inhibits in vitro porcine embryo development

The CatSper channel modulates boar sperm motility during capacitation

Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows

Membrane Stress During Thawing Elicits Redistribution of Aquaporin 7 But Not of Aquaporin 9 in Boar Spermatozoa

Immunolocalization of E-cadherin and β-catenin in the cyclic and early pregnant canine endometrium

Will AI in pigs become more efficient?

The ubiquitous hyaluronan: Functionally implicated in the oviduct?

Non-viable sperm in the ejaculate: Lethal escorts for contemporary viable sperm

The role of placental-decidual interactions in promoting fetal tolerance

Flow Cytometric Chromosomal Sex Sorting of Stallion Spermatozoa Induces Oxidative Stress on Mitochondria and Genomic DNA

Boar Differences In Artificial Insemination Outcomes: Can They Be Minimized?

The activity of paraoxonase type 1 (PON‐1) in boar seminal plasma and its relationship with sperm quality, functionality, and in vivo fertility

Boar spermatozoa successfully predict mitochondrial modes of toxicity: Implications for drug toxicity testing and the 3R principles

Effect of Overnight Staining on the Quality of Flow Cytometric Sorted Stallion Sperm: Comparison with Tradtitional Protocols

Colloid Centrifugation Selects Normal Spermatozoa from Polymorphic Bull Ejaculates: A Case Study

Sperm chromatin structure and sperm morphology: Their association with fertility in AI-dairy Ayrshire sires

Sex sorting increases the permeability of the membrane of stallion spermatozoa

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