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Purpose. To improve the viability of the 2/4/A1 cell culture model and to investigate different routes of drug transport in this cell line. Methods. Two approaches were taken to decrease apoptosis. First, rat intestinal 2/4/A1 cells were transfected to overexpress the antiapoptotic protein Bcl-2. Second, normal 2/4/A1 cells were cultivated under conditions that stimulate differentiation and...
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