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The genome is partitioned into regions of euchromatin and heterochromatin. The organization of heterochromatin is important for the regulation of cellular processes such as chromosome segregation and gene silencing, and their misregulation is linked to cancer and other diseases. We present a model-based approach for automatic 3D segmentation and 3D shape analysis of heterochromatin foci from 3D confocal...
We introduce a 3D model-based approach for automatic segmentation of 3D fluorescent heterochromatin foci from microscopy images. The approach employs a new 3D parametric intensity model based on a spherical harmonics (SH) expansion and can represent foci of regular and highly irregular shapes. By solving a least-squares minimization problem, the new model is directly fitted to the 3D image data, and...
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