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Regulation of neurotransmitter release in the central nervous system is complex. Here, we investigated regulatory mechanisms for acetylcholine (ACh) release from cholinergic neurons by performing superfusion experiments with rat striatal segments after labelling the cellular ACh pool with [3H]choline. Electrical stimulation‐evoked pronounced [3H]ACh release from cholinergic neurons. The estimated...
In addition to hydrolysis by acetylcholine esterase (AChE), acetylcholine (ACh) is also directly taken up into brain tissues. In this study, we examined whether the uptake of ACh is involved in the regulation of synaptic ACh concentrations. Superfusion experiments with rat striatal segments pre‐incubated with [3H]choline were performed using an ultra‐mini superfusion vessel, which was developed to...
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