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We demonstrate the cellular imaging of holographic fluorescence microscopy by implementing self-interference incoherent digital holography (SIDH) apparatus. Complex holograms of three different wavelengths are acquired and post processed to generate pseudo color image of cell.
Digital holography is an emerging technology in biomedical microscopy that yields high-sensitivity high-resolution quantitative images of cellular microscopic structures and processes. An overview of recent research and development of digital holographic microscopy will be presented.
We discuss and demonstrate our recent developments using self-interference incoherent digital holography to bridge the gap between indispensable incoherent imaging techniques and the advantages of three-dimensional holographic recording. We demonstrate the ability to record broadband sunlit photographic holograms, apply non-invasive three-dimensional tracking techniques, and the ease of adaptation...
We achieve three-dimensional photography of continuous objects and scenery under ambient (sunlight) illumination based on self-interference of two beam-split copies of the object's optical field with differential curvatures. The apparatus consists of a beam-splitter, a few mirrors and lenses, a piezo-actuator, and a CCD camera. No lasers or other special illumination is used for recording or reconstruction...
Terahertz digital off-axis holography is demonstrated with a highly coherent, frequency tunable continuous wave terahertz source emitting between 0.1 and 0.8 THz and a single spatially-scanned Schottky diode detector. The angular spectrum and dual wavelength reconstruction methods are employed for the digital reconstruction process.
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