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A PCR-based subtractive cloning procedure was used to identify genes expressed at higher levels in the pancreatic beta cell line βTC1, as compared to the pancreatic alpha cell line αTC1. One of the clones isolated by this procedure corresponded to the regulatory subunit (RIα) of protein kinase A (PKA). Using antibodies directed against RIα, we now demonstrate both by immunoblot and immunofluorescence...
The complex anatomy of the mammalian pancreas, in which the endocrine cells are grouped in islets dispersed among the predominant exocrine component, has hampered study of the molecular events governing the development of pancreatic cell lineages. To investigate whether fish may provide relevant, complementary models of pancreas development, we characterized the trout insulin (tINS) promoter and its...
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