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Recent progress in single DNA manipulation with atomic force microscope has proved its potential in analysing genetic information at the molecular level instead of macro ensemble approach. However, current manipulation of DNA is mainly carried out by manual operations, which is labour‐intensive and time‐consuming, thus limits the further applications in other fields. In this paper, an optimized DNA...
We demonstrate a simple technique for the sample preparation of cells cultured on nanostructure patterns for microscopic imaging. Conventional cell fixation technique was used for the preparation of template. Then, soft lithography was applied to produce negative replica. If necessary, positive replica could be obtained by a second imprinting with negative replica as mold. For illustration, Hela cells...
When live endothelial cells imaged using atomic force microscopy (AFM), distortion of the cell shape could be caused by the interaction between the probe and cell membrane, which depends on the force and the scanning speed used. In this study, a shape recovering method was developed. Two forces were considered in the method: the vertical and the lateral force of the tip exerted on the cell membrane...
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