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Somatic embryo quality is still a problem for many researchers. To improve the efficiency of germination, special procedures are used, such as partial drying of somatic embryos at high relative humidity or desiccation in the presence of supersaturated solutions of salt. In this work, cotyledonary somatic embryos of Norway spruce (Picea abies) and Serbian spruce (P. omorika) were placed on culture...
The process of soil degradation and destabilization of forest ecosystem by industrial pollution is frequently associated with mobilization of toxic Al³⁺ ions. Both these processes exert a negative influence on tree root systems and may even result in the decline of whole forest stands. One-year-old seedlings of silver birch (Betula pendula Roth.) grown in pots were treated with a range of aluminum...
From May till October 1999, 1-year-old birch seedlings were grown in a greenhouse in a substrate (forest soil under a birch tree + perlite, 1:1), without and with added aluminium chloride (40 and 160 mg Al dm⁻³). The added aluminium chloride inhibited the growth of seedlings, especially of their roots. At the end of the experiment the substrate treated with aluminium chloride contained more Al and...
Adventitious bud cultures were established by using buds of selected poplar clones (Populus tremula L. × P. alba L.) as initial explants. The Murashige and Skoog medium (1/2 and 1/4 MS) was used for multiplication and rooting of shoots. To the media, aluminium was added in the form of sulphate, at a concentration 50−70 mg Al dm-3. The culture was continued in vitro for more than 12 months. The cultures...
Adventitious bud cultures were established by using buds of selected poplar clones (Populus tremula L. × P. alba L.) as initial explants. The Murashige and Skoog medium (1⁄2 and 1⁄4 MS) was used for multiplication of shoots. Aluminium and copper were added to the medium in the form of sulphates. Copper, lead and aluminium were also added to the medium in the form of nitrates. Low concentrations of...
One-year-old seedlings of silver birch (Betula pendula Roth.) were grown in pots filled with a soil substrate that originated from an area polluted by a phosphate fertiliser factory and characterised by a high soil Al level and low Ca/Al ratio or with a substrate from an area regarded as free from toxic pollution. In addition the effect of fertilisation with a mixture of nutrients was evaluated. Birch...
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