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Murashige & Skoog nutrient was supplemented with substances of molecular weight (MW) less than 5 kDa, which were separated from extract of winter wheat ears by means of Sephadex G-25 ultrafiltration. Isolated embryos of the same wheat cultivar (Grana) were vernalized in the nutrient for 0 and 7 days at 2 °C for 2 weeks and planted in a glass-house. After 150 days of growth (20/17 °C day/night)...
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