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Scrapie is a fatal, neurodegenerative disease occurring in sheep, goats and mufflons. It is commonly believed that its infectious agent is a protease-resistant form of host-encoded prion protein (PrP). Polymorphism of the coding region of PrP genes has been analyzed in many countries. Polymorphism of codons 136, 154 and 171 has been associated with outbreaks of scrapie. Valine (V) in codon 136, arginine...
Epizootiological studies have shown that meat-and-bone meal of ovine or bovine origin added to feed concentrates are infectious agents in bovine spongiform encephalopathy (BSE). In view of the ban on the use of meat-and-bone meal in animal feed in European Union countries, the aim of the study was to elaborate methods for identifying chicken and sheep DNA in compound feeds. A duplex PCR reaction (simultaneous...
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