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One of the critical factors restricting the progress in crop breeding and genetic studies is the time required in generating desired ‘pure line’ segregating populations. We developed a procedure that can advance up to seven generations of canola per annum, allowing the production of pure line populations within a year. An ‘all soil’ method was compared with an ‘embryo culture plus soil’ procedure...
Generating segregating populations and ‘pure lines’ is an essential component in many projects of plant breeding and biological studies. Time taken to obtain these materials is often a critical factor restricting the progress of such projects. We report a procedure here which, by combining embryo culture with managements of watering regimes, lighting intensity and duration, temperature and quantity...
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