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The aim of this study was to perform molecular analysis of canine adenovirus 2 (CAV-2) E1B 19K gene fragment isolated from 20 dogs of various breeds (12 males and 8 females aged 1-9 years), with clinical symptoms of upper respiratory tract infections, from the Lubelszczyzna region. Nasal swabs were taken from dogs. DNA of CAV-2 was detected using the PCR method in 16 swabs. All PCR products were sequenced,...
The aim of the present study was to investigate the occurrence of Anaplasma spp. in group of 50 fallow deer (Dama dama) from free-range farm in eastern Poland and determine what species of Anaplasma could infect these animals based on PCR gene sequencing. The PCR technique revealed the presence of 16S RNA Anaplasma spp. genetic material in the blood of 7 out of 50 examined animals. The sequences of...
The aim of this study was to use the real time polymerase chain reaction in the detection of Babesia canis subclinical infestations in dogs and to compare the different DNA isolation methods on PCR sensitivity. The study included 6 dogs with suspected subclinical babesiosis. DNA for real time polymerase chain reaction were isolated by the phenol method as well as by Micro AX Gravity (A & A Biotechnology,...
The aim of this study was to assess the safety of a new vaccine, containing soluble parasitic antigen (SPA), against canine babesiosis. Fifteen dogs were included in the experiment. Five controls received only the adjuvant and 10 dogs were vaccinated with Babesia canis canis SPA twice, at a two weeks interval. For the whole period of the study all animals were under constant clinical observation....
This study was aimed at determining the cause of the diseases in five horses exhibiting symptoms of fever, joint oedema and ataxia and thrombocytopenia. The PCR technique revealed the presence in the blood of 16S RNA Anaplasma/Ehrlichia spp. genetic material. DNA amplification with primers EHR 521 and EHR 747 gave a product with a size of 247 bp. The sequence of the PCR product obtained showed a 97...
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