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High concentrations of 422 g sophorolipids l−1 were produced using a two-stage cultivation process: first deproteinized whey concentrate (DWC) containing 110 g lactose l−1 was used for cultivation of the yeast Cryptococcus curvatus ATCC 20509, resulting in 34 g dry weight l−1, 20 g single-cell oil l−1 and reducing the chemical oxygen demand (COD) from 159 g l−1 to 35 g oxygen l−1. Afterwards cells...
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