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We present a study of dynamic atomic force microscopy (AFM) in liquid medium. In the first part, we investigate hydrodynamic viscous damping. Using an experimental analysis of the thermal noise motion of the cantilever, the dependency of the hydrodynamic damping versus the cantilever–surface separation was evaluated. In the second part, we present an improvement of the excitation holder that allows...
Loss of stability and integrity of large membrane protein complexes as well as their aggregation in a non-lipidic environment are the major bottlenecks to their structural studies. We have tested C12H25-S-poly-Tris-(hydroxymethyl)acrylamidomethane (H12-TAC) among many other detergents for extracting the yeast F1F0 ATP-synthase. H12-TAC was found to be a very efficient detergent for removing the enzyme...
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