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The acute effects of low concentrations of ethanol on intracellular free magnesium ions ([Mg 2+ ] i ) in cultured type-2 astrocytes were studied by digital imaging microscopy using the Mg 2+ fluorescent probe, mag-fura-2. In 0-mM ethanol, the basal level of [Mg + ] i was 124.7± 2.56 μM with a heterogeneous distribution within the cells. Treatment of the cells...
Ethanol ingestion can cause irreversible neuronal and vascular damage in the brain and stroke-like events. Using an intact in vivo rat brain (pial) model, TV image-intensification, cultured cerebral vascular muscle cells, digital-image analysis, and a novel Mg 2+ ion-selective electrode to measure extracellular ionized Mg 2+ , studies were designed to determine whether:...
Acute effects of a series of alcohols (methanol, ethanol, n-butanol) on intracellular free magnesium concentration ([Mg 2+ ] i ) in canine cerebral vascular smooth muscle cells was studied using mag-fura-2 and digital imaging microscopy. In 1.2 mM [Mg 2+ ] o , basal [Mg 2+ ] i was 500 ± 30 μM. Exposure of cells to a low concentration...
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