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This paper presents a simple and reliable method for separating the anodic peaks of dopamine (DA) and ascorbic acid (AA). Good electrochemical responses of DA and AA can be obtained at an electrochemically pretreated glassy carbon electrode (PGCE). The electrode reaction of DA is a quasi-reversible process, but the oxidation of AA is entirely irreversible. Consequently, the PGCE can be used to separate...
. The interaction of indophenol blue (IPB) with proteins in aqueous solution has been studied by optical absorption and Rayleigh light scattering (RLS) spectroscopy. At pH 3.8, the weak RLS of IPB is enhanced by proteins. Based on this phenomenon, a novel method for the determination of proteins at nanogram levels using the RLS technique is developed. The method is simple, practical and sensitive...
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