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We have previously shown that, in vivo, the integration system based on the gene encoding the TG1 integrase and the corresponding attBTG1 and attPTG1 sites works well not only in Streptomyces strains, but also in Escherichia coli. Furthermore, the attachment sites for TG1 integrase are distinct from those of ϕC31 integrase. In this report, we expressed TG1 integrase as a GST-TG1 integrase fusion...
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