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Subcellular fractions from developing seeds of mustard (Sinapis alba), honesty (Lunaria annua) and nasturtium (Tropaeolum majus) synthesize very long chain cis (n-9) monounsaturated fatty acids, e.g. gadoleic (20:1), erucic (22:1) and nervonic (24:1) acid, from oleoyl-CoA and malonyl-CoA by condensation reactions. The particulate 2000 x g and 15 000 x g fractions exhibit considerably higher elongase...
Membrane-bound enzymes involved in oleate metabolism in microsomes from pea (Pisum sativum L.) leaves were solubilised using detergents, such as n-octyl glucoside, Triton X-100, digitonin or cholate. The detergents were found to be inhibitory to oleoyl-CoA thioesterase, oleoyl-CoA:phosphatidylcholine acyltransferase and oleoyl phosphatidylcholine desaturase. Detergent removal by dialysis resulted...
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