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Various adenine nucleotides activated phospholipase C of FRTL-5 cell membranes in the following order of activity, ATPγS > ATP > AppNp > AppCp = ADP > MeSATP. This order was well consistent with that observed in intact cells. Such activation occurred only in the presence of appropriate concentrations of GTPγS and Ca 2+ , in a way similar to the norepinephrine-induced activation...
Norepinephrine (NE) stimulated FRTL-5 thyroid cells via an α 1 -adrenergic receptor, resulting in cytosolic Ca 2+ ([Ca 2+ ] i ) mobilization and activation of phospholipase C. Adenosine and its receptor agonist, phenylisopropyladenosine (PIA), although not exerting a direct effect, markedly enhanced the NE-induced changes. Basal NE action was not totally abolished...
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