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2 ,3 -Di-O-tert-butyldimethylsilyl-4 -C-(hydroxymethyl)uridine was synthesized and converted into the phosphoramidite building blocks 9 and 13. Novel oligodeoxynucleotide analogues containing 4 -C-hydroxymethyl linked phosphodiester internucleoside linkages and 3 -hydroxyl linked phosphodiester internucleotide linkages were synthesized on an automated DNA-synthesizer. The latter modification...
Automated synthesis of novel mono-branched ( Y-shaped ) and double-branched ( H-shaped ) oligodeoxynucleotide analogues has been accomplished by use of phosphoramidite chemistry. The synthetic strategy involves stereoselective 3 -O-phosphitylation of nucleoside 2 to give building block 3 and fully automated stereoselective elongation and subsequent branching of amidite 3 on a DNA-synthesizer...
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