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Background: β-lactam antibiotic therapies are commonly challenged by the hydrolytic activities of β-lactamases in bacteria. These enzymes have been grouped into four classes: A, B, C, and D. Class B β-lactamases are zinc dependent, and enzymes of classes A, C, and D are transiently acylated on a serine residue in the course of the turnover chemistry. While class A and C β-lactamases have been extensively...
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