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Pyridoxine 5′-phosphate oxidase (PNP Ox) catalyzes the terminal step in the biosynthesis of pyridoxal 5′-phosphate. The 53-kDa homodimeric enzyme contains a noncovalently bound flavin mononucleotide (FMN) on each monomer. Three crystal forms of Escherichia coli PNP Ox complexed with FMN have been obtained at room temperature. The first crystal form belongs to trigonal space group P3 1 21 or...
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