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Effective proteome profiling is generally considered to depend heavily on the availability of a high‐quality DNA reference database. As such, proteomics has long been taxonomically restricted, with limited inroads being made into the proteomes of “non‐model” organisms. However, next generation sequencing (NGS), and particularly RNA‐Seq, now allows deep coverage detection of expressed genes at low...
The isolation and analysis of glycoproteins by coupling lectin affinity chromatography with MS has emerged as a powerful strategy to study the glycoproteome of mammalian cells. However, this approach has not been used extensively for the analysis of plant glycoproteins. As with all eukaryotes, N‐glycosylation is a common post‐translational modification for plant proteins traveling through the secretory...
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