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We have developed a fully automatable two‐dimensional liquid chromatography platform for shotgun proteomics analyses based on the online coupling of hydrophilic interaction liquid chromatography (HILIC) – using a nonionic type of TSKgel Amide 80 at either pH 6.8 (neutral) or 2.7 (acidic) – with conventional low‐pH reversed‐phase chromatography. Online coupling of the neutral‐pH HILIC and reversed...
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