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Sterile cultivated plant cell tissues and cell regenerates of several species were tested for their binding affinity to the central human benzodiazepine receptor. Binding activity was found in extracts of Artemisia dracunculus cell tissue (IC 50 = 7 μg/ml) and, to a lesser extent, in plant regenerates of potato herb (Solanum tuberosum). Preparative HPLC led to the isolation of fractions with...
A sensitive and selective method to determine diazepam, N-desmethyldiazepam, nitrazepam, flunitrazepam and medazepam in human serum and urine was established employing solid-phase extraction and high-performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-ESI-MS-MS) with the selected reaction monitoring (SRM) mode. Using diazepam-d 5 and N-desmethyldiazepam-d 5 ...
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