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Cell suspension cultures of Catharanthus roseus were elicited with an autoclaved cell-free filtrate of Pythium aphanidermatum. The regulation of alkaloid, terpenoid, and phenolic biosynthetic pathways was studied by product formation and assay of the enzyme activity of anthranilate synthase (AS), tryptophan decarboxylase (TDC), strictosidine synthase (SSS), strictosidine-β-glucosidase (SG), isopentenyl...
The possible limitation of the rate of biosynthesis of terpenoid indole alkaloids by low enzyme levels was investigated in two cell lines of Tabernaemontana divaricata with different terpenoid indole alkaloid biosynthetic capacities. The activities of tryptophan decarboxylase (TDC), strictosidine synthase (SSS), strictosidine glucosidase (SG), isopentenyl pyrophosphate isomerase (IPP isomerase) and...
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