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The sub-cellular feruloylation and oxidative coupling sites of cell wall polysaccharides were investigated in planta by monitoring the kinetics of appearance of arabinosyl- and feruloyl-radiolabelled polysaccharides in the protoplasmic compartment and their secretion in the wall either in the presence or absence of brefeldin A (BFA). By using root apical segments excised from wheat seedlings (Triticum durum...
Two new reducing glycoconjugates [N-d-galacturonoyl-putrescinamide (GalA–Put) and N,N′-di-d-galacturonoyl-putrescinamide (GalA–Put–GalA)] and homogalacturonan–putrescine (GalA n –Put) conjugates were synthesised as model compounds representing possible amide (isopeptide) linkage points between a polyamine and either one or two pectic galacturonate residues. The amide bond(s) were stable to...
The in vivo changes in the growth and in the biosynthesis of cell wall polysaccharides (pectins, hemicelluloses and α-cellulose) were studied in apical and subapical root segments isolated from water stressed and unstressed wheat seedlings (Triticum durum Desf.) cv. Capeiti 'drought tolerant' and cv. Creso 'drought sensitive'. In both cultivars, water stress imposed by a 20% solution of polyethylene...
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