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Although a cell culture system for HCV JFH‐1 strain has been developed, no robust cell culture system for serum‐derived HCV is available. In this study, we have established systems capable of monitoring infection with JFH‐1 virus based on specific reporter gene expression through proteolysis of chimeric transcription factors by HCV NS3/4A protease. We utilized a transcriptional factor Gal4‐TBP that...
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