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To extend the range of biofuel cell applications, we wish to increase their maximum operational temperatures. Using a thermostable alcohol dehydrogenase as a biocatalyst, we prepared an enzyme‐immobilized bioanode that can operate at high temperatures. The catalytic current for ethanol oxidation was increased using this electrode at temperatures up to 80 °C.
In this communication, we describe a novel and facile method for the immobilization of NAD + /NADH on an electrode surface using a hydrophobic ionic liquid, 1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ([C4mim][Tf 2 N]). By taking advantage of the insolubility of NAD + /NADH in hydrophobic ionic liquids, it is expected that NAD + /NADH can be retained...
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