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Loop 6 in the active site of Triosephosphate Isomerase (Saccharomyces cerevisiae) moves in order to reposition key residues for catalysis. The timescale of the opening and closing of this loop has been measured, at temperatures from −15 to +45°C, using broadline solid state deuterium NMR of a single deuterated tryptophan located in the loop’s N terminal hinge. The rate of the loop opening and closing...
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