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In situ transesterification of Chlorella alga was performed using 5–20wt% sulfuric acid as a catalyst at either 60 or 100°C. The maximum ester yield in the range of 96–98% is comparative to the specification of ester content in biodiesel, 96%. A high excess of methanol was used in transesterification ensured also a high ester yield. The FAME was purified via adsorption of chlorophyll and carotenoids...
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