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The role of protein disulfide isomerase (PDI) in the regeneration of ribonuclease A with dithiothreitol (DTT) was investigated at three different temperatures. The rates of formation of the native protein were markedly increased in the presence of PDI, 9-fold at 15 o C, 6-fold at 25 o C and 62-fold at 37 o C, respectively. In the presence of PDI, major changes were found in...
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