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A new type of DNA ligand that contains a phosphate‐binding group and a photoresponsive azobenzene moiety is reported. When the azobenzene is in trans configuration, the ligand binds to the minor groove of a double‐stranded DNA, whereas it partially desorbs upon trans–cis isomerisation with light. The ability to photoswitch the ligand upon interaction with DNA is evidenced by (chir)optical signatures,...
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