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''Positive control'' mutants of the cI protein of bacteriophage λ (λcI) bind DNA but, unlike the wild-type protein, fail to activate transcription. According to the original interpretation of Ptashne and co-workers, these mutants bear amino acid substitutions that disrupt a stimulatory interaction between λcI bound at operator site O R 2 and RNA polymerase bound at promoter P RM ...
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