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A new synthesis of the nucleoside analogue 3-deaza-2'-deoxycytidine is described. The N 3 -nitrogen of dC is involved in the central hydrogen bond of a dG-dC base pair. The relative importance of hydrogen bonding as a controlling factor in the activity of DNA polymerase is examined by studying both duplex stability and template effects for this dc 3 C nucleoside. Duplexes containing...
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