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Metal-chelated supermacroporous cryogels are effective supports for affinity chromatographic separation of biomolecules in downstream processes. In this work, polyacrylamide cryogel beds were prepared in glass columns (16mm inner diameter) and coupled with iminodiacetic acid (IDA). These cryogels were loaded with Zn 2+ and Ni 2+ and the so-called Zn 2+ -IDA-cryogels and Ni...
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