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We report a method to prepare a DNA–enzyme conjugate using histidine-tag (His-tag) chemistry. A DNA oligonucleotide was modified with nitrilotriacetate (NTA), whose K d was approximately 10 −6 (M −1 ) toward a His-tag present on a recombinant protein via the complexation of Ni 2+ . His-tagged alkaline phosphatase (His-AP) was used as the model enzyme. Enzyme immobilization...
We present the DNA-assisted control of enzymatic activity for the detection of a target protein using a new type of DNA–enzyme conjugate. The conjugate is composed of an enzyme inhibitor to regulate enzyme activity and a DNA aptamer to be responsive toward the analyte protein. Glutathione S-transferase (GST) and thrombin were selected as a model enzyme and an analyte protein. A hexahistidine tag was...
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