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To introduce the carbonyl group to sugars in a continuous process, pyranose 2-oxidase was immobilized on a poly(vinylidene fluoride) membrane by hydrophobic interaction, and sugar solutions were passed through the membrane. The amount of immobilized pyranose 2-oxidase reached 14mgg −1 in 1h in permeation mode. With increase in the space velocity of the solution of one of the sugars used (sucrose),...
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