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Let the Fur fly: Mutation of a single glutamate into an aspartate was shown to make the Fe sensor Fur as reactive to H2O2 as the peroxide sensor PerR. In vivo and in vitro peroxide sensitivities of a series of PerR and Fur Asp/Glu mutants were studied by mass spectrometry. A combination of Mössbauer spectroscopy analyses and DFT calculations gave a structural rationale for this behavior.
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