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Two fenthion haptens, 4-(4-(dimethoxyphosphorothioyloxy)-2-methylphenylamino)-4-oxobutanoic acid (H1) and 6-(methoxy(4-(methylthio)phenoxy)phosphorothioylamino)hexanoic acid (H2), were synthesized. H1 was conjugated with bovine serum albumin (BSA) and H2 with ovalbumin (OVA) by the active ester method. Then H2-OVA conjugate was used as coating antigen, while H2-BSA conjugate was used to produce polyclonal...
Five haptens with different spacer-arm attachment sites on the structure of the organophosphorus insecticide fenthion were designed and synthesized. All of the haptens were conjugated with ovalbumin (OVA) for the coating antigen, and three haptens containing all or most of the structure of fenthion were conjugated with bovine serum albumin (BSA) for the immunogen. Six polyclonal antisera were raised...
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