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We report on initial results of using a new direct detection device (DDD) for single particle reconstruction of vitreous ice embedded specimens. Images were acquired on a Tecnai F20 at 200keV and a nominal magnification of 29,000×. This camera has a significantly improved signal to noise ratio and modulation transfer function (MTF) at 200keV compared to a standard CCD camera installed on the same...
The voltage dependence characteristics of thin-foil based phosphor screens in the thickness range of ~ 10-60 μm are examined for CCD imaging in transmission electron microscopy (TEM) in the voltage range of 80-400 kV. The brightest screen is obtained with a P20 layer of about 12 μm at 80 kV, and a thicker screen lowers both the screen brightness and resolution. The thickness of the brightest screen...
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