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Dopamine (DA) is one of catecholamines and related to several neurological diseases. The selective determination for DA against other catecholamines is crucial in clinical diagnoses. In this work, a simple and reliable Tb(III)-based fluorescence sensor was constructed for the highly selective and sensitive detection of DA. Silver nanoprisms (AgNPrs) with suitable localized surface plasmon resonance...
It was found that silver nanoparticles (AgNPs) could enhance co-luminescence effect of rare earths ions Tb3+ and Y3+. Based on this, a sensitive fluorescence detection method for the determination of dopamine (DA) was proposed. Moreover, the detection limit for DA was very low (down to nM). This is because DA can remarkably enhance the luminescence intensity of the Tb3+ ion by Y3+ in the colloidal...
Nucleic acids can greatly enhance fluorescence intensity of the kaempferol (Km)–Al(III) system in the presence of silver nanoparticles (AgNPs). Based on this, a novel method for the determination of nucleic acids is proposed. Under studied conditions, there are linear relationships between the extent of fluorescence enhancement and the concentration of nucleic acids in the range of 5.0×10 −9 ...
A new method for detecting protein by synchronous fluorescence enhancement was developed, based on the combination of near infrared (NIR) fluorescence and the dedimerization phenomenon of methylene blue (MB). Under analytical conditions, there are linear relationships between the enhancing extent of synchronous fluorescence of MB–sodium dodecyl benzene sulfonate (SDBS)–protein at 667nm and the concentration...
It is found that Al(III) can further enhance the intensity of resonance light scattering (RLS) of the silver nanoparticles (AgNPs) and nucleic acids system. Based on this, a novel method of determination of nucleic acids is proposed in this paper. Under optimum conditions, there are linear relationships between the enhancing extent of RLS and the concentration of nucleic acids in the range of 1.0×10...
This paper reported the coupling technique of Ag island film-enhanced fluorescence with rare earth co-luminescence effect of Tb–Gd–sodium dodecyl benzene sulfonate (SDBS)–protein system. While the collagen is used as the separator between Ag island film and the fluorophore because it not only can decrease the fluorescence of the blank, but also can promote the adsorption of other proteins and change...
A novel method is proposed in this paper, that is the silver nanoparticle (nanoAg)–cetyltrimethylammonium bromide (CTMAB) is used as the probe of resonance light scattering (RLS) for the determination of nucleic acids. Under optimum conditions, there are linear relationships between the quenching extent of RLS and the concentration of nucleic acids in the range of 4.0×10 −9 –2.0×10 −6...
A new fluorimetric method for the determination of epinephrine is established by rare earth co-luminescence effect of Tb–Gd–E–Tris system. Under optimum conditions, a linear relationship has been obtained between the fluorescence intensity and the concentration of epinephrine in the range of 1.8×10 −8 to 2.5×10 −6 mol/l, and the detection limit is 4.5×10 −9 mol/l (S/N=3). The...
It is found that the fluorescence intensity of morin–Al 3+ complex can be greatly enhanced by proteins in the presence of cetyltrimethylammonium bromide (CTAB). It is considered that protein and CTAB provide a hydrophobic environment with low polarity and large viscosity, resulting in the fluorescence enhancement of morin–Al 3+ complex. The experiments indicate that under optimum conditions,...
It is found that the fluorescence of Tb-adenosine triphosphate (ATP)-phenanthroline (phen) system can be enhanced by Gd 3+ . The fluorescence enhancement of the Tb-Gd-ATP-phen system is considered to originate from intramolecular and intermolecular energy transfers, and the energy-insulating sheath effect of Gd-ATP-phen complex. In addition, a new energy transfer pathway in Tb-ATP-phen...
It is found that Tb 3+ can react with tryptophan (Trp) and sodium dodecyl benzene sulfonate (SDBS), and emits the intrinsic fluoresence of Tb 3+ . The fluorescence intensity can be enhanced by La 3+ , Gd 3+ , Lu 3+ , Sc 3+ and Y 3+ , among which Gd 3+ has the greatest enhancement. This is a new...
It is found that the fluorescence intensity of Tb-cetylpyridine bromide (CPB)-nucleic acid system can be enhanced by La 3+ , Gd 3+ , Lu 3+ , Sc 3+ and Y 3+ , of which Gd 3+ has the greatest enhancement. The experiments indicate that under the optimum condition, the fluorescence intensity of the system is in proportion to...
A simple and sensitive fluorimetric method for the determination of ascorbic acid (AA) is described. The method is based on the condensation reaction between AA and o-phenylenediamine (OPDA) in the absence of the oxidant. The fluorescence intensity is measured at excitation and emission wavelengths of 360 and 430 nm, respectively. Under optimum condition, a linear relationship is obtained between...
A new resonance light scattering (RLS) assay of proteins is presented. In the citric acid-NaOH (pH 2.35) buffer, the RLS of Resorcinol yellow (RY)-protein system can be greatly enhanced by addition of nonionic surfactant OP, owing to the interaction between OP and RY-protein. The enhanced RLS is in proportion to the concentration of proteins in the range 0.02-4.0 μg ml -1 for both bovine...
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