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The role of acrylamide on the human peripheral lymphocytes was studied by laser scanning confocal microscopy technique and fluo-3. The calibration value of the apparent dissociation constant (K d ) of the fluo-3-Ca 2+ complex was obtained as 4.83×10 −7 mol/L. Acrylamide (<54μg/mL) evoked a rise in free intracellular calcium concentration [Ca 2+ ] i , in a...
Characteristic of Fura-2-Ca 2+ interaction was studied based on the fluorescence technique. The apparent dissociation constants (K d ) of the Fura-2-Ca 2+ complex were determined at different temperature. The effect of cefotaxime (CEFA) on intracellular Ca 2+ concentration ([Ca 2+ ] i ) was discussed by using a ratiometric fluorescence dye Fura-2 as...
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