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The purpose of this study was to develop a microscopy-based foci quantification protocol in human lymphocyte capable of supplying useful data for radiation sensitivity assays. Human peripheral blood was exposed to gamma-rays and isolated lymphocytes were stained with fluorochrome-coupled anti-gamma-H2AX (Histone 2AX phosphorylation of serine 139) antibodies. Microscopy slides were automatically acquired...
This paper considers how well standard calibration curve for translocations constructed for lymphocyte cultures irradiated in vitro with gamma-rays from 60 Co compares with the translocations yield in lymphocytes taken from people at a long post-exposure time. Data were used from radiation accident victims overexposed to doses ranging from 0.2 to 8.5 Gy and who were cytogenetically followed-up...
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