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Maintenance of cellular number and functions of attached hepatocytes during culture is an important requirement for hepatocyte-based drug screening applications. In this study, two carboxymethylcellulose (CMC)-based hydrogels were used as hepatocyte culture substrata to improve the stability of cellular adhesiveness, bioactivity and hepatic function of primary rat hepatocytes. The hydrogels were prepared...
An aqueous solution of carboxylmethylcellulose with phenolic hydroxyl groups (CMC-Ph) is gellable within 1min via a peroxidase-catalyzed oxidative reaction under mild conditions suitable for mammalian cells. In this research, we evaluated cellular adhesion and proliferation on the resultant hydrogel, and the feasibility of the hydrogel as a substrate for cell sheet technology. Within 4h of seeding,...
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