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An ORF2 gene located upstream of the cellulose synthase (bcs) operon of Acetobacter xylinum BPR2001 was disrupted and a mutant (M2-2) was constructed. In static cultivation, the parent strain produced a tough, colorless, and insoluble cellulose pellicle, whereas M2-2 culture produced a thin, yellow, and fragile pellicle. The results of X-ray diffraction and 13 C solid-state NMR indicated...
Acetobacter xylinum, which normally produces ribbon-like microfibrils of cellulose I, occasionally synthesizes a band-like cellulose (native band, or NB) having a cellulose II crystal structure with a putative folded-chain structure. In contrast to a previous finding of NB production by a mutant strain of A. xylinum, we found that the wild-type strain also produced NB when incubated on agar plate...
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