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A new method to obtain enzymatic rate coefficients for synthesis of biological homopolymers is presented. The new method involves fitting molecular weight distributions (MWDs) obtained by taking samples at successive times from an in vitro enzyme-catalyzed system. Enzymatic rate coefficients can then be obtained by fitting the observed MWDs to an assumed model. These types of results are complementary...
The structure of branched polysaccharides involves a hierarchy of levels, from the constituent sugars, then the branching pattern, up to the macromolecular architecture, and then supramolecular organization. Finding causal relations between this complex structure/architecture and both (bio)synthetic mechanisms and final properties is needed for understanding the functionality of branched polysaccharides,...
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